Optimasi Proses Biodegumming Serat Rami (Boehmeria nivea) Menggunakan Bakteri Pektinolitik

AbstrakProses degumming serat rami umumnya dilakukan menggunakan senyawa alkali dalam jumlah besar, sehingga dihasilkan limbah berlebih dan berakibat pada pencemaran lingkungan. Oleh sebab itu, metode alternatif dengan memanfaatkan agensia hayati untuk mengatasi permasalahan tersebut penting dikembangkan. Penelitian ini bertujuan melakukan seleksi kandidat bacterial dari kulit batang serta optimasi biodegumming isolat bakteri pektinolitik. Bakteri pektinolitik diisolasi yang terkomposkan media pektinase (PSAM). Isolat diperoleh kemudian digunakan sebagai beberapa parameter meliputi pH (8,5 9), suhu (25 °C 37,5 °C), jenis (Pe-Ku 1, Pe-Ku 4, 6) inokulum (1/50 mL, 2/50 3/50 mL). Setelah inkubasi selama 3 hari, dicuci ditimbang bobot kering mengetahui penurunan bobotnya. Analisis statistik two-way ANOVA uji lanjut Duncan. Hasil penelitian menunjukan bahwa telah 9 degumming. Berdasarkan kondisi optimum yaitu 8,5; °C; mL mengggunakan 6. Hal ditandai secara signifikan lebih tinggi dibandingkan kombinasi perlakuan lain, sebesar 12,7%. Kata kunci: degumming; pektinolitik; Kulit rami; Serat AbstractThe process of ramie fiber is generally carried out by using large amounts alkaline compounds, causing excessive waste and environmental pollution. Therefore, it essential to develop alternative methods utilizing biological agents overcome these problems. This study aimed select candidates for from bark optimize the method pectinolytic isolates. Pectinolytic bacteria were isolated composted pectinase screening agar medium The isolates then used as several optimization parameters, including (8.5 9.0), temperature 37.5 type isolate 1; 4; Pe- Ku amount inoculum After incubation three days, was washed, measured dry weight determine reduction. statistical analysis performed with Duncan post hoc test. results showed that nine obtained agents. In addition, based on method, conditions at 8.5, °C, 6 isolate. That indicated percentage loss, which significantly higher than other combination treatments,